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  • Histone H3 threonine 11 phosphorylation is catalyzed directly by the meiosis-specific kinase Mek1 and provides a molecular readout of Mek1 activity in vivo

    Authors
    Kniewel, Ryan
    Murakami, Hajime
    Liu, Yan
    Ito, Masaru
    3 more author(s)...
    Description

    Summary from the GEO: "Saccharomyces cerevisiae Mek1 is a CHK2/Rad53-family kinase that regulates meiotic recombination and progression upon its activation in response to DNA double-strand breaks (DSBs). The full catalog of direct Mek1 phosphorylation targets remains unknown. Here, we show that phosphorylation of histone H3 on threonine 11 (H3 T11ph) is induced by meiotic DSBs in S. cerevisiae and...

    Subject
    Checkpoint Kinase 2
    Chromatin Immunoprecipitation Sequencing
    Histones
    Phosphorylation
    Protein-Serine-Threonine Kinases
    Access Rights
    Free to All

  • DNA damage-induced transcriptome changes in budding yeasts Saccharomyces cerevisiae and Candida glabrata

    Authors
    Shor, Erika
    Perlin, David S.
    Description

    Summary from the GEO: "This study compared the transcriptional response to DNA damage in two closely related yeasts, S. cerevisiae and C. glabrata." Overall design from the GEO: "We cultured S. cerevisiae and C. glabrata in YPD containing 0.1% methyl methanesulfonate (MMS) for 1 hour, harvested the cells, isolated total RNA using the Qiagen Rneasy kit, and sent the RNA to Genewiz for RNAseq. Untreated...

    Subject
    Checkpoint Kinase 2
    DNA Damage
    Proliferating Cell Nuclear Antigen
    Access Rights
    Free to All